Preparation process of β-Nicotinamide Mononucleotide
preparation process of β-Nicotinamide Mononucleotide is summarized as follows:
first, β-Nicotinamide Mononucleotide nicotinamide ribose salt is phosphorylated under alkaline conditions to obtain nicotinamide mononucleotide salt, β-Nicotinamide Mononucleotide and then acidified under acidic conditions to obtain the target initial product, and then After the purification step, β-Nicotinamide Mononucleotide the pure product of the target product is obtained.
The reaction formula is as follows:
The preparation method of β-nicotinamide mononucleotide specifically includes the following steps:
phosphorylation reaction, β-Nicotinamide Mononucleotide in an alkaline aqueous solution of pH 8-10, the temperature of the reaction system is Under the condition of 25-50°C, β-Nicotinamide Mononucleotide the nicotinamide ribose salt with the formula structure and the metaphosphate are subjected to a phosphorylation reaction for 2-5 hours to obtain an intermediate product with the structure of the formula iii.
Acidification reaction:
add acid to the reaction solution, β-Nicotinamide Mononucleotide adjust the pH value to 3 to 5, and lower the temperature of the reaction system, β-Nicotinamide Mononucleotide acidify the intermediate product with formula structure to form β-nicotinamide mononucleotide with formula i structure; product purification.
The reaction solution after step b is filtered through protein membrane, concentrated by high-pressure desalting membrane, separated and purified by anion exchange resin, β-Nicotinamide Mononucleotide and concentrated and crystallized to obtain pure β-nicotinamide mononucleotide.
Preferably, β-Nicotinamide Mononucleotide in step a, m in the formula is sodium or potassium, metaphosphate is sodium metaphosphate or potassium metaphosphate, β-Nicotinamide Mononucleotide and the content of sodium trimetaphosphate in metaphosphate is calculated as p2o5, and the content is greater than 68%, β-Nicotinamide Mononucleotide 1 The ph of% aqueous solution is 6.2.
The alkaline aqueous solution used is any one of sodium hydroxide, potassium hydroxide, β-Nicotinamide Mononucleotide lithium hydroxide, calcium hydroxide and magnesium hydroxide aqueous solution.
The pH value of the reaction system is 9, β-Nicotinamide Mononucleotide the reaction temperature is preferably 30°C, and the reaction time is 4 hours.
Through online reaction monitoring, β-Nicotinamide Mononucleotide the raw materials are consumed after 4 hours of reaction.
*Special note - This article is for informational purposes only and cannot replace a doctor's treatment diagnosis and advice. It should not be regarded as a recommendation or proof of efficacy of the medical products involved. If it involves disease diagnosis, treatment, and rehabilitation, please be sure to go to a professional medical institution to seek professional advice.
by GSHWORLD
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