Effects of glutathione on the growth and development of cucumber seedlings

 

Glutathione (GSH) is the main and most abundant thiol-containing low molecule widely distributed in plant and microbial cells. It is an effective enzyme activity regulating substance and plays an important role in improving the drought resistance, low temperature stress resistance and salt stress resistance of plants. In addition, plants produce a large amount of endogenous GSH in vivo under heavy metal stress, which can remove heavy metal ions in cells and play a detoxification role. It has been shown that some dinitroaniline herbicide treatments can increase the content of GSH and oxidized glutathione (GSSG) in the root tissues of tomato and melon plants.

The researchers used cucumbers as materials and applied exogenous GSH to study the effects of exogenous glutathione on the growth, development and physiology of cucumber seedlings under atrazine soil residues, in order to alleviate the effects of glutathione on cucumber plants. oxidative stress, reducing the damage caused by atrazine soil residues, and improving the resistance of cucumber plants to provide references.

The test results showed that after exogenous glutathione treatment, the GSH content in the leaves increased, and the GR enzyme activity and GSTs enzyme activity increased simultaneously, which was similar to the mitigation effect of carnation seedlings under cadmium poisoning. After exogenous GSH treatment, the chlorophyll and net photosynthetic rate of the seedlings increased, and the leaf area and fresh weight of cucumber seedlings were significantly affected.

Corn, sorghum, sugarcane and other crops contain a glutathione transferase, which can promote the formation of water-soluble complexes of atrazine and glutathione, and make atrazine inactive in these crops, thereby making These crops are not harmed. Therefore, there is not enough glutathione transferase in the crops not treated with exogenous GSH, and atrazine shows higher activity after being absorbed by these crops and inhibits their photosynthesis, making these crops more vulnerable to damage.

The GSH content, GR activity and GSTs activity of cucumber seedlings treated with exogenous GSH increased, so the chlorophyll content and net photosynthetic rate of cucumber seedlings increased compared with the control, and the leaf area and fresh weight per plant increased significantly, thereby Increase dry matter accumulation of cucumber, among them, 100 mg/L exogenous glutathione treatment had the greatest effect on seedlings. This is similar to the research results that exogenous GSH can relieve autotoxicity and inhibit the growth of pepper seedlings.

In general, the treatment of exogenous GSH with appropriate mass concentration can reduce the damage of soil atrazine to cucumber seedlings, increase the activity of plant antioxidant enzymes and photosynthesis of seedlings, thereby improving the growth of crops. The comprehensive test factors show that 100 Exogenous GSH at mg/L had the best mitigation effect on cucumber seedlings under atrazine stress in soil.

Original text: Luwen, SONG Shuyao, CHEN Shanshan, et al.Effects of Exogenous Glutathione on Growth and Development of Cucumber Seedlings under Atrazine Stress[J].JOURNAL OF JILIN AGRICULTURAL UNIVERSITY,2023,45(03):279-284.)

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